PhotoshopForums.com Home
Navigate Contact FAQ Search Members
photoshop for science...need some help
Post new topic   Reply to topic    PhotoshopForums.com Forum Index -> Manipulation
 See a User Guidelines violation? Please contact us.
Author Message

willmer

Joined: 23 Mar 2012
Posts: 2



PostPosted: Fri Mar 23, 2012 10:16 am    Post subject: photoshop for science...need some help Reply with quote

Hello, I am a biology researcher who has very minimal photoshop experience. I often take pictures of cells and focus on their nuclei, the part of the cell that holds DNA. When I take pictures with our microscope, often there is a lot of background color that makes it difficult to distinguish the nuclei. Unfortunately, our microscope does not quite work for the kind of imaging I'm doing but I would like to see if its possible to salvage the images I have already taken. When I try to edit the levels in PS, I can manage a slightly better picture, but is there a way to take the pictures I have included that are black and white and make them as clear as the picture I attached with the blue nuclei?


FG-TP3 BF648B5 OS - DMSO-0096_c3.JPG
 Description:
This is my original image from the microscope. Instead of clear differences between a black background and blue/white nuclei, it is all different shades of grey.
 Filesize:  95.74 KB
 Viewed:  429 Time(s)

FG-TP3 BF648B5 OS - DMSO-0096_c3.JPG



DAPI nuclei.jpg
 Description:
This is what I want my images to look like. Clear distinction between the nuclei (blue) and a black background. The nuclei here have been pseudo colored blue.
 Filesize:  15.72 KB
 Viewed:  429 Time(s)

DAPI nuclei.jpg


View user's profile Send private message

thehermit

Joined: 05 Mar 2003
Posts: 3987
Location: Cheltenham, UK


PostPosted: Fri Mar 23, 2012 11:24 am    Post subject: Reply with quote

Not really, this is the best I could do. If you want the details of how I did it I can email or dropbox the PSD to you, failing that I may post a breakdown with images (easier to post the PSD).

As I say, it's not really the same, but its ermm blue! :)



cells.jpg
 Description:
 Filesize:  24.08 KB
 Viewed:  421 Time(s)

cells.jpg



_________________
If life serves you lemons, make lemonade!
View user's profile Send private message

willmer

Joined: 23 Mar 2012
Posts: 2



PostPosted: Fri Mar 23, 2012 11:41 am    Post subject: Reply with quote

Thanks for the attempt. Its not the blue color that interests me as much, but rather the distinction between the nuclei and the background. I would love to see the PSD tho and figure out what changes you made. My emails is soccerislife61488 "at" yahoo.com
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    PhotoshopForums.com Forum Index -> Manipulation All times are GMT - 6 Hours
Page 1 of 1
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum
You cannot attach files in this forum
You can download files in this forum


Contact - User Guidelines >

Copyright © 2003-2016. PhotoshopForums.com, iFroggy Network. All Rights Reserved.
Powered by phpBB © phpBB Group. phpBB SEO. Privacy Policy.
We are in no way affiliated with Adobe. Photoshop, Adobe and related marks are registered trademarks of Adobe.
PhotoshopForums.com